Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity

By M. Khalil, E. H. Ismail, K. EL-BAGHDADY, D. Mohamed. 2014

789 citations recorded by the source corpus.

Abstract The silver nanoparticles (AgNPs) synthesized using hot water olive leaf extracts (OLE) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against drug resistant bacterial isolates. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles are investigated. The data revealed that the rate of formation of the nanosilver increased significantly in the basic medium with increasing temperature. The nature of AgNPs synthesized was analyzed by UV–vis spectroscopy, X-ray diffraction, scanning electron microscopy and thermal gravimetric analysis (TGA). The silver nanoparticles were with an average size of 20–25 nm and mostly spherical. The antibacterial potential of synthesized AgNPs was compared with that of aqueous OLE by well diffusion method. The AgNPs at 0.03–0.07 mg/ml concentration significantly inhibited bacterial growth against multi drug resistant Staphylococcus aureus ( S. aureus ), Pseudomonas aeruginosa ( P. aeruginosa ) and Escherichia coli ( E. coli ). This study revealed that the aqueous olive leaf extract has no effect at the concentrations used for preparation of the Ag nanoparticles. Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future.

Subfields: Electronic, Optical and Magnetic Materials, Materials Chemistry, Plant Science

View Paper: https://www.semanticscholar.org/paper/b1c47ae061be53f9baed254fe6b26f3f4a711fdb

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Citations in the corpus

A review on green synthesis of silver nanoparticles and their applications (2017, background): “…by UV–Vis spectroscopy, XRD and SEM as well as revealed their effective antibacterial activity towards Staphylococcus aureus and Shigella sp. Khalil et al. (2014) studied that Ag-NPs are synthesized by the reduction of AgNO3 solution through olive leaf extract and these particles showed…”
Green Synthesis of Silver Nanoparticles: A Review (2016, background): “The above stated mechanism is in agreement with the reports of many authors [64] [72] [75]”
A Review on the Green Synthesis of Silver Nanoparticles and Their Morphologies Studied via TEM (2015, background): “Figure 7: TEMmicrograph of the silver nanoparticles [48]: (a) at pH 3, nm and (b) at pH 8 (reproduced with permission from the journal).”
Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity (2016, background): “It has been mentioned (Khalil et al., 2013) that theabsorbanceof theAgNPs obtained fromolive leave extract increaseswith increasing pH of the solution from 2 to 8.”
Green synthesis of nanoparticles and its potential application (2015, positive): “The authors confirmed that the produced NPs possess good antibacterial activity against drug resistance bacterial isolates (Khalil et al. 2014).”
Influence of temperature and concentration on biosynthesis and characterization of zinc oxide nanoparticles using cherry extract (2018, background): “The major effect of pH is changing the electrical charges of biomolecules which might change their reducing and capping ability and then the growth of nanoparticles [40].”
Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract (2016, background): “…Silambarasan, & Abraham, 2012), Spirogyra varians (Salari, Danafar, Dabaghi, & Ataei, 2014), Melia dubia (Ashokkumar et al., 2013), olive (Khalil, 2013), leaf extract of Acalypha indica with high antibacterial activities (Krishnaraj et al., 2010) and of Sesuvium portulacastrum also…”