Considerations for the design and reporting of enzyme assays in high-throughput screening applications

By M. Acker, D. Auld. 2014

182 citations recorded by the source corpus.

Abstract This review describes the key steps and methods which are used to develop enzyme assays suitable for high-throughput screening (HTS) applications. The goals of HTS enzyme assays are defined relative to lower-throughput bench top assays and important aspects which go into constructing robust and sensitive enzyme assays are described. Methods that have been applied to common enzyme classes are reviewed and pitfalls related to assay artifacts are discussed. We also suggest a reporting format to describe the steps in HTS enzyme assays.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/b1b23dd8e7b8266872ce61bfe456d2a62c603848

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In Molecular Biology

1. Hak-Sung Kim
2. J. Choi
3. Sang-Soo Han
4. John M. Woodley
5. R. Sheldon

In Spectroscopy

1. Hak-Sung Kim
2. J. Choi
3. Sang-Soo Han

Citations in the corpus

• Industrial applications of enzyme biocatalysis: Current status and future aspects. (2015, methodology): “To use the focused-directed evolution, high-throughput screening (HTS) system with high sensitivity and efficiency is crucial (Acker and Auld, 2014; Kazlauskas, 2008).”