Three-dimensional organotypic culture: experimental models of mammalian biology and disease
By E. Shamir, Andrew J. Ewald. Nature reviews. Molecular cell biology, 2014
671 citations recorded by the source corpus.
Mammalian organs are challenging to study as they are fairly inaccessible to experimental manipulation and optical observation. Recent advances in three-dimensional (3D) culture techniques, coupled with the ability to independently manipulate genetic and microenvironmental factors, have enabled the real-time study of mammalian tissues. These systems have been used to visualize the cellular basis of epithelial morphogenesis, to test the roles of specific genes in regulating cell behaviours within epithelial tissues and to elucidate the contribution of microenvironmental factors to normal and disease processes. Collectively, these novel models can be used to answer fundamental biological questions and generate replacement human tissues, and they enable testing of novel therapeutic approaches, often using patient-derived cells.
Subfields: Biomedical Engineering, Cell Biology, Oncology
View Paper: https://www.semanticscholar.org/paper/b12b7ea979a44ad032a4abd3494d33b8ba7426c4
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biomedical Engineering
In Cell Biology
In Oncology
Citations in the corpus
- Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning (2018, background): “Over the past decade, the term organoid has broadened and now encompasses a variety of tissue culture techniques that result in self-organizing, self-renewing 3D cultures derived from primary tissue, embryonic stem cells, or induced pluripotent stem cells that have a similar functionality as the tis”