Production and structural characterization of surfactin (C14/Leu7) produced by Bacillus subtilis isolate LSFM-05 grown on raw glycerol from the biodiesel industry
By A. F. Faria, Diego Stéfani Teodoro-Martinez, Geraldo Nazareno de Oliveira Barbosa, B. Vaz, Ísis Serrano Silva, J. Garcia, M. Tótola, M. Eberlin, M. Grossman, O. L. Alves and 1 more. 2011
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Abstract The production of biosurfactant by Bacillus subtilis LSFM-05 was carried out using raw glycerol, obtained from a vegetable oil biodiesel plant in Brazil, as the sole carbon source. Production of the biosurfactant was carried out in a 15-L bench-top fermentor and the surfactant was obtained from the foam produced. The crude surfactant was purified by silica gel column chromatography with a yield of 230 mg of the purified biosurfactant per liter of foam. TLC, IR spectroscopy, 1 H and 13 C NMR and Fourier transform ion cyclotron resonance mass spectrometry with electrospray ionization (ESI-FTMS) were used to characterize the purified surfactant. The isolated surfactant was identified as a surfactin lipopeptide. MS/MS data identified the amino acid sequence as GluOMe-Leu-Leu-Asp-Val-Leu-Leu and showed that the fatty acid moiety contained 14 carbons in iso , anteiso or normal configurations. The critical micelle concentration of the C 14 /Leu 7 surfactin was 70 μM, with emulsification efficiency after 24 h ( E 24 ) of 67.6% against crude oil. Raw glycerol represents an abundant and renewable carbon source and provides an opportunity for reducing the cost of biosurfactant production and may add value to biodiesel production by creating new commercial applications for this by-product.
Subfields: Molecular Biology, Pollution
View Paper: https://www.semanticscholar.org/paper/b109768dcf521727799f311d771d96d7d955d4a9
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Citations in the corpus
- Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications (2013, background): “This first FTIR-ATR analysis indicates the presence of aliphatic hydrocarbons combined with a peptide moiety that is characteristic of lipopeptide biosurfactants, as it has been previously described in the literature [34,36].”