Reconstruction of the neuromuscular junction connectome

By Ranga Srinivasan, Ju Lu, Jeff William Lichtman. Bioinform., 2010

3,153 citations recorded by the source corpus.

Motivation: Unraveling the structure and behavior of the brain and central nervous system (CNS) has always been a major goal of neuroscience. Understanding the wiring diagrams of the neuromuscular junction connectomes (full connectivity of nervous system neuronal components) is a starting point for this, as it helps in the study of the organizational and developmental properties of the mammalian CNS. The phenomenon of synapse elimination during developmental stages of the neuronal circuitry is such an example. Due to the organizational specificity of the axons in the connectomes, it becomes important to label and extract individual axons for morphological analysis. Features such as axonal trajectories, their branching patterns, geometric information, the spatial relations of groups of axons, etc. are of great interests for neurobiologists in the study of wiring diagrams. However, due to the complexity of spatial structure of the axons, automatically tracking and reconstructing them from microscopy images in 3D is an unresolved problem. In this article, AxonTracker-3D, an interactive 3D axon tracking and labeling tool is built to obtain quantitative information by reconstruction of the axonal structures in the entire innervation field. The ease of use along with accuracy of results makes AxonTracker-3D an attractive tool to obtain valuable quantitative information from axon datasets. Availability: The software is freely available for download at http://www.cbi-tmhs.org/AxonTracker/ Contact: stwong@tmhs.org

View Paper: https://www.semanticscholar.org/paper/b0f27b13b14c557401c8b3b2a6eff4384b22f0c0

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Citations in the corpus

Mercator: a fast and simple web server for genome scale functional annotation of plant sequence data. (2014, methodology): “A range of popular tools implement this approach (GOblet, GOtcha, Blast2GO, KAAS; Martin et al. 2004; Moriya et al. 2007; Conesa & Gotz 2008; Groth et al. 2008).”
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