SIGNATURE: a single-particle selection system for molecular electron microscopy.

By James Z. Chen, N. Grigorieff. 2007

173 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Structural Biology, Surfaces, Coatings and Films

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Citations in the corpus

A Primer to Single-Particle Cryo-Electron Microscopy (2015, unknown): “…with classification (Hall and Patwardhan, 2004; Lata et al., 1995); and those that employ templates, i.e., either class averages of particles selected from micrographs or projections from a known 3D structure of the complex (Chen and Grigorieff, 2007; Huang and Penczek, 2004; Sigworth, 2004).”
A structural model of the active ribosome-bound membrane protein insertase YidC (2014, methodology): “The defocus was determined using the TF ED command in SPIDER followed by automated particle picking using Signature (Chen and Grigorieff, 2007).”
Structural Dynamics of the YidC:Ribosome Complex during Membrane Protein Biogenesis (2016, methodology): “Single particles were picked from final 1,792 micrographs using the automated SIGNATURE software (Chen and Grigorieff, 2007) using ten representative projections of a 70S ribosome as references.”
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA (2015, unknown): “Particles were detected with SIGNATURE (Chen and Grigorieff, 2007).”
DeepPicker: a Deep Learning Approach for Fully Automated Particle Picking in Cryo-EM (2016, methodology): “The generative approaches [11, 12, 13] usually measure the similarity to a reference to identify particle candidates from micrographs.”
DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy. (2009, methodology): “The SIGNATURE particle picking package implements a process in which the FLCF is further supplemented with an additional cross-correlation, the spectral correlation, that reinforces the distinctiveness of peaks corresponding to correct matches of the template (Chen & Grigorieff 2007).”