Production and purification of lentiviral vectors

By G. Tiscornia, O. Singer, Inder M. Verma. Nature Protocols, 2006

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Cell type-specific profiling of protein-DNA interactions without cell isolation using Targeted DamID with next-generation sequencing (2007, neutral): “A detailed protocol describing a highly similar method on how to produce purified and titrated lentiviruses can be found in ref”
Synaptic signaling by all-trans retinoic acid in homeostatic synaptic plasticity (2008, positive): “Lentivirus was produced as described (Tiscornia et al., 2006).”
Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons (2010, methodology): “Concentrating by ultracentrifugation The ultracentrifugation procedure used here evolved from a lentiviral protoco”
Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats. (2009, methodology): “LV stocks were produced by calcium phosphate transfection into 293T cells, as previously described (Follenzi and Naldini 2002; Tiscornia et al. 2006).”
Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132. (2010, positive): “Viral particles were packaged using a third-generation system (Tiscornia et al., 2006).”
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase (2009, background): “2 (gag/pol) (plasmid 8455) and transfer vector expressing Cre (modified from plasmid 17408)) ( Addgene”