A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae.

By Wei Chen, Chengwu Zhang, Lirong Song, M. Sommerfeld, Q. Hu. Journal of Microbiological Methods, 2009

720 citations recorded by the source corpus.

Subfields: Molecular Biology, Renewable Energy, Sustainability and the Environment

View Paper: https://www.semanticscholar.org/paper/1c6ff4de47f159faeec35afa919b3e660f148457

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Citations in the corpus

The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella (2011, positive): “Nile red signals were captured using an excitation wavelength of 480 nm, and emission was collected between 560 and 600 nm (Huang et al., 2009; Chen et al., 2009).”
Use of sodium bicarbonate to stimulate triacylglycerol accumulation in the chlorophyte Scenedesmus sp. and the diatom Phaeodactylum tricornutum (2012, methodology): “1987), which has been correlated with cellular TAG concentrations (Chen et al. 2009; Cooksey et al. 1987; Elsey et al. 2007; Lee et al. 1998).”
Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves (2011, background): “reinhardtii based on Nile red is available [45] but is mainly qualitative.”
Improved Nile Red staining of Nannochloropsis sp. (2011, methodology): “The use of DMSO to assist NR staining has been previously reported by Simonetti et al. (1995) and Chen et al. (2009) .”
A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels (2011, background): “Microalgae like Chlorella, Dunaliella, Chlamydomonas, Scenedesmus, and Spirulina are known to contain a large amount (>50% of the dry weight) of starch, cellulose and glycogen, which are raw materials for ethanol production [64,65].”
Effect of nitrogen concentration on growth, lipid production and fatty acid profiles of the marine diatom Phaeodactylum tricornutum (2017, methodology): “The lipid concentration was determined using Nile red measurement (Hitachi f-2700 fluorescence spectrophotometer at EX 530 and EM 575) according to Chen et al. (2009).”
Isolation of high-lipid content strains of the marine microalga Tetraselmis suecica for biodiesel production by flow cytometry and single-cell sorting (2011, background): “Other authors have also reported highly significant correlations between the NR fluorescence and total microalgal lipid content (Chen et al. 2009; Cooksey et al. 1987; de la Jara et al. 2003; Lopes da Silva et al. 2009), thus supporting the view that the dye is a useful proxy for the quantification…”
A high throughput Nile red fluorescence method for rapid quantification of intracellular bacterial polyhydroxyalkanoates (2013, methodology): “The efficacy of Nile red to stain the lipids was further improved when the staining conditions regarding the staining temperature, incubation time and dye concentration were optimized to obtain the optimum condition for the lipid staining in microalgae [10].”
Microalgae Isolation and Selection for Prospective Biodiesel Production (2012, background): “Dimethyl sulfoxide (DMSO) has also been used for enhancing lipid staining effectiveness [33,34,37] and maybe used in low quantities instead of acetone as a solvent to allow viability of cells after staining.”