Heterologous protein expression in the methylotrophic yeast Pichia pastoris.

By J. L. Cereghino, J. Cregg. FEMS Microbiology Reviews, 2000

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A Pichia pastoris fermentation strategy for enhancing the heterologous expression of an Escherichia coli phytase (2004, background): “Some earlier studies have demonstrated that the gradual addition of methanol prior to the glycerol depletion in the culture medium might have facilitated the de-repression of the AOX1 promoter[24,27].”
Heterologous protein production using the Pichia pastoris expression system (2005, methodology): “pastoris expression system is to obtain maximum amounts of expressed foreign proteins [18].”