Centrifugation Assisted Agrobacterium tumefaciens-mediated Transformation (CAAT) of embryogenic cell suspensions of banana (Musa spp. Cavendish AAA and Lady finger AAB)

By H. Khanna, D. Becker, Jennifer Kleidon, J. Dale. Molecular breeding, 2004

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Factors affecting somatic embryogenesis and transformation in modern plant breeding (2010, background): “In banana cell suspensions, althoug high inoculum densities (1.0 and 2.0) resulted in hgh transient gene expression, cell death was also high (Khanna et al., 2004) indicating that densities giving high transient expression may not translate into a high number of stable transformants.”
Improved frequency of transformation in rice and maize by treatment of immature embryos with centrifugation and heat prior to infection with Agrobacterium tumefaciens (2006, background): “The mechanisms behind such an improvement are not known but seem to be somewhat different from the observations of Khanna et al. (2004) .”