Maximizing DNA loading on a range of gold nanoparticle sizes.

By Sarah J. Hurst, Abigail K. R. Lytton-Jean, Chad A. Mirkin. Analytical Chemistry, 2006

1,076 citations recorded by the source corpus.

We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to approximately 0.7 M NaCl in the presence of DNA containing a poly(ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have approximately 2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.

Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/acd291afb251aa3386d1dd483b942312b58e4fe3

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In Biomedical Engineering

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Citations in the corpus

A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise (2015, background): “In bottom to top approach, chemical reduction is the most common scheme for syntheses of silver nanoparticles [8,9].”
A review on green synthesis of silver nanoparticles and their applications (2017, methodology): “The tube furnace used in this method has various drawbacks as it occupies a substantial space and munches a lot of energy while raising temperature around the source material, and it similarly entails a lot of time to succeed thermal stability (Hurst et al. 2006; Elghanian et al. 1997).”
Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap. (2011, positive): “DNA-modified AuNPs (DNA sequence: 3′-HS-(CH 2 ) 3 -A 10 -PEG 18 -AAACTCTTTGCGCAC-5′) were prepared according to literature procedure”
Techniques for physicochemical characterization of nanomaterials (2013, background): “Furthermore, conjugation of an extrinsic fluorophore to the non-intrinsically fluorescent nanomaterials enables FS to determine the characteristics of biomolecule on the NP surface, including concentration, particle size, and spacer composition (Hurst et al., 2006).”
Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles (2010, background): “…to the molecule of interest, the presence of those molecules on the particle surface can be accessed by the absorption or fluorescence emission (Demers et al. 2000; Meiser et al. 2004; Sari et al. 2004; Hurst et al. 2006), or by stepwise photobleaching of the organic dye (Casanova et al. 2007).”