Sensing protein molecules using nanofabricated pores
By Anpan Han, G. Schürmann, G. Mondin, R. Bitterli, Nicole G. Hegelbach, N. F. de Rooij, U. Staufer. 2006
177 citations recorded by the source corpus.
We report the detection of protein molecules with nanofabricated pores using the resistive pulse sensing method. A 20-nm-thick silicon nitride membrane with a nanofabricated pore measuring about 55nm in diameter separated an electrolyte cell into two compartments. Current spike trains were observed when bovine serum albumin (BSA) was added to the negatively biased compartment. The magnitude of the spikes corresponded to particles 7–9nm in diameter (the size of a BSA molecule) passing through the pore. This suggests that the current spikes were current blockages caused by single BSA molecules. The presented nano-Coulter counting method could be applied to detect single protein molecules in free solution, and to study the translocation of proteins through a pore.
Subfields: Biomedical Engineering, Computational Mechanics
View Paper: https://www.semanticscholar.org/paper/acb80a73c415769e17496b2f2264246fa8623437
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biomedical Engineering
In Computational Mechanics
Citations in the corpus
- Solid-state nanopores. (2007, background): “It is also possible to translocate non-polynucleotide polymers, in particular to observe the passage of single protein molecule”