Microfluidics for exosome isolation and analysis: Enabling liquid biopsy for personalized medicine

By Jose C. Contreras-Naranjo, Hung-Jen Wu, V. Ugaz. Lab on a Chip, 2017

454 citations recorded by the source corpus.

Exosomes, the smallest sized extracellular vesicles (30 ~ 150 nm) packaged with lipids, proteins, functional messenger RNAs and microRNAs, and double-stranded DNA from their cells of origin, have emerged as key players in intercellular communication. Their presence in bodily fluids, where they protect their cargo from degradation, makes them attractive candidates for clinical application as innovative diagnostic and therapeutic tools. But routine isolation and analysis of high purity exosomes in clinical settings is challenging, with conventional methods facing a number of drawbacks including low yield and/or purity, long processing times, high cost, and difficulties in standardization. Here we review a promising solution, microfluidic-based technologies that have incorporated a host of separation and sensing capabilities for exosome isolation, detection, and analysis, with emphasis on point of care and clinical applications. These new capabilities promise to advance fundamental research while paving the way toward routine exosome-based liquid biopsy for personalized medicine.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ac92bb473e02fe00fe3ae413b211a92053f7f1f4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

In Molecular Biology

Citations in the corpus

Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics (2020, background): “This is especially valuable for non-invasive disease detection, such as early-stage cancer screening [114, 115].”
A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option? (2020, background): “For a comprehensive review of microfluidics-based exosome isolation, please refer to this excellent review [82].”
Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes (2019, background): “Microfluidics-based immunoaffinity capture (Mf-IAC) uses “capture antibodies” or “capture beads” targeting specific surface markers of EV subpopulations [90].”