Definitions and guidelines for research on antibiotic persistence
By Nathalie Q. Balaban, S. Helaine, Kim Lewis, Martin Ackermann, B. Aldridge, D. Andersson, M. P. Brynildsen, D. Bumann, A. Camilli, James J. Collins and 15 more. Nature Reviews Microbiology, 2019
1,144 citations recorded by the source corpus.
Increasing concerns about the rising rates of antibiotic therapy failure and advances in single-cell analyses have inspired a surge of research into antibiotic persistence. Bacterial persister cells represent a subpopulation of cells that can survive intensive antibiotic treatment without being resistant. Several approaches have emerged to define and measure persistence, and it is now time to agree on the basic definition of persistence and its relation to the other mechanisms by which bacteria survive exposure to bactericidal antibiotic treatments, such as antibiotic resistance, heteroresistance or tolerance. In this Consensus Statement, we provide definitions of persistence phenomena, distinguish between triggered and spontaneous persistence and provide a guide to measuring persistence. Antibiotic persistence is not only an interesting example of non-genetic single-cell heterogeneity, it may also have a role in the failure of antibiotic treatments. Therefore, it is our hope that the guidelines outlined in this article will pave the way for better characterization of antibiotic persistence and for understanding its relevance to clinical outcomes. Antibiotic persistence contributes to the survival of bacteria during antibiotic treatment. In this Consensus Statement, scientists working on the response of bacteria to antibiotics define antibiotic persistence and provide practical guidance on how to study bacterial persisters.
Subfields: Genetics, Molecular Medicine
View Paper: https://www.semanticscholar.org/paper/ac0f0949604fc5e7891c1dc78ac0808adb48cd16
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In Genetics
- M. Huemer
- George M. Church
- S. Brugger
- James J. Collins
- Annelies S. Zinkernagel
- Srikanth Mairpady Shambat
- Dongbin Xiu
- Karthik Devarajan
In Molecular Medicine
- M. Huemer
- S. Brugger
- Eric D. Brown
- James J. Collins
- David L. Paterson
- Annelies S. Zinkernagel
- Srikanth Mairpady Shambat
Citations in the corpus
- A Deep Learning Approach to Antibiotic Discovery. (2020, background): “Next, we considered whether halicin might induce bacterial cell death against E. coli in a metabolically repressed, anti-biotic-tolerant state (Balaban et al., 2019; Stokes et al., 2019a, 2019b).”
- Biofilms as Promoters of Bacterial Antibiotic Resistance and Tolerance (2020, background): “Indeed, persistence is a phenomenom that increase the survival of a given population in the presence of bactericidal antibiotics without enhancing the MIC, but in contrast to tolerance, persistence only affects a subset of cells of the population called persisters [64].”
- Antibiotic resistance and persistence—Implications for human health and treatment perspectives (2020, positive): “…Persistence and Antimicrobial Therapy”, 121 scientists studying antibiotic persistence and tolerance came together in Ascona, Switzerland, and laid the foundations for a “Consensus Statement” on the definitions and guidelines for research on antibiotic tolerance (Balaban et al , 2019a, b).”
- Antimicrobial Resistance in ESKAPE Pathogens (2020, background): “For example, IS-mediated deactivation of the ompK36 porin in K. pneumoniae results in elevated carbapenem MICs (281).”