A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems.

By Nicholas J. Agard, Jennifer A. Prescher, Carolyn R. Bertozzi. Journal of the American Chemical Society, 2004

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Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation (2014, methodology): “Among the many recent productive uses of SPAAC methodology are the derivatization of purified proteins in vitro, (Agard et al., 2004), glycans on cell surfaces for probing sialic acid biosynthesis (Beatty et al., 2010), and in vivo experiments with zebrafish (Dehnert et al., 2012), the C. elegans…”