MIFlowCyt: The Minimum Information about a Flow Cytometry Experiment

By Jamie A. Lee, Josef Spidlen, K. Boyce, Jennifer Cai, N. Crosbie, M. Dalphin, Jeff Furlong, M. Gasparetto, Michael Goldberg, Elizabeth M. Goralczyk and 24 more. Cytometry Part A, 2008

483 citations recorded by the source corpus.

A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross‐disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third‐party understanding and reuse of FCM data. © 2008 International Society for Advancement of Cytometry

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/aba0b5a294dbdbe7f6300bdb05e846d8b70a44c8

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Citations in the corpus

• MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments (2020, methodology): “Furthermore, we recommend that the ISAC MIFlowCyt guidelines checklist is completed and reported for all EV-FC experiments, as well as to report experimental preanalytical variables related to basic flow cytometric equipment and reagents [3].”
• Cytobank: providing an analytics platform for community cytometry data analysis and collaboration. (2014, methodology): “Cytobank Reports meet existing flow cytometry data standards, including MIFlowCyt, created by the International Society for Analytical Cytology and American Chemical Society Analytical Cytometry Standards (Society 2013, Lee et al. 2008).”
• Computational flow cytometry: helping to make sense of high-dimensional immunology data (2016, methodology): “FlowRepository is a public repository that supports deposition, querying and downloading of data that is annotated according to the MIFlowCyt (Minimal Information about a Flow Cytometry experiment) 23 compliance format.”