Design of a genome-wide siRNA library using an artificial neural network

By Dieter Huesken, Joerg Lange, C. Mickanin, J. Weiler, F. Asselbergs, J. Warner, Brian Meloon, Sharon Engel, Avi Rosenberg, D. Cohen and 4 more. Nature Biotechnology, 2005

388 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ab946b4ec58b29827e4cca5aa0b1611ca8838a8f

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Citations in the corpus

The promises and pitfalls of RNA-interference-based therapeutics (2009, neutral): “One of these, an artificial neural network, has been used to develop a genome-wide siRNA library for humans and to identify effective siRNAs for 34 target”
Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, methodology): “This is consistent with endonucleases preferring to slice after U, the complementary base to A (Huesken et al., 2005; Reynolds et al., 2004).”
Highly integrated single-base resolution maps of the epigenome in Arabidopsis. (2008, neutral): “Thus, we observe a tendency for a TA dinucleotide that would provide an optimal motif for cleavage of a double-stranded RNA species by the DICER-LIKE3 endonuclease (Huesken et al., 2005; Reynolds et al., 2004).”
On the art of identifying effective and specific siRNAs (2006, background): “The evolving understanding of the RNAi mechanism, together with statistical analyses of libraries of siRNAs with experimentally determined efficiency, led to computer-based approaches that increased the likelihood of identifying effective and specific siRNA”
3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets (2006, background): “These studies have led to the development of multiple design algorithms that enhance the selection of highly functional duplexe”