Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples

By David S. Pilliod, Caren S. Goldberg, Robert S. Arkle, L. Waits. 2013

581 citations recorded by the source corpus.

Subfields: Ecological Modeling, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ab806fbb04c12d002405feeadd3a1cb32da782f2

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Citations in the corpus

Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity (2015, background): “River Crustacean, mussel Switzerland Present Pilliod et al. (2014) Factors influencing detection of eDNA from a streamdwelling amphibian Streams Amphibian (idaho giant salamander) Idaho, USA Present Tréguier et al. (2014) Environmental DNA surveillance for invertebrate species: advantages and techni”
Characterizing the distribution of an endangered salmonid using environmental DNA analysis (2015, methodology): “Any samples that showed signs of inhibition (delayed or non-amplification of internal controls) were diluted to 0.1 to reduce inhibition and re-run in triplicate (Pilliod et al., 2013).”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, methodology): “…I (COI) DNA (e.g. Yu et al. 2012; Ji et al. 2013; Machler et al. 2014), whereas mitochondrial cytochrome b (cyt-b) gene regions were the most frequent target for the detection and identification of amphibians (e.g. Goldberg et al. 2011; Olson et al. 2012; Pilliod et al. 2013; Spear et al. 2015).”
Meta‐analysis supports further refinement of eDNA for monitoring aquatic species‐specific abundance in nature (2019, background): “Although contamination is a potential issue (Pilliod et al., 2013), standardizing qPCR curves from plasmids can allow researchers to directly estimate copy count number present in eDNA, bypassing this potential issue.”
Taxonomic and geographical representation of freshwater environmental DNA research in aquatic conservation (2019, methodology): “For species presence– absence data, eDNA methods have been shown to outperform traditional biodiversity surveys (Hänfling et al., 2016; Pilliod et al., 2013) .”
Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels (2017, methodology): “, 2017), filters (Pilliod et al., 2013), or extraction methods (Lakay et al.”
Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data (2015, methodology): “2002, 2006), but recent work showed that SOMs can be successfully applied to the analysis of eDNA data (Pilliod et al. 2013; Schmidt et al. 2013).”
Transport Distance of Invertebrate Environmental DNA in a Natural River (2014, background): “They either found no relationship [16,31] or a positive relationship [15,17,18].”
Particle size distribution and optimal capture of aqueous macrobial eDNA (2014, background): “2) and others (Jerde et al. 2011; Dejean et al. 2012; Thomsen et al. 2012a; Pilliod et al. 2013) suggests an extensive search mode (i.”
How will the ‘molecular revolution’ contribute to biological recording? (2015, methodology): “Little hard data exists on the DNA shedding rates of organisms (but see Pilliod et al., 2013 for an exception) but knowledge of DNA persistence in different environments is increasing.”