Electroporation and RNA interference in the rodent retina in vivo and in vitro

By T. Matsuda, C. Cepko. Proceedings of the National Academy of Sciences of the United States of America, 2003

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Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery. (2005, background): “injection (33, 34), others have shown that siRNA has activity in vivo using delivery in viral vectors (8, 9), retinal electroporation (35), and direct intracellular (36), intratumoral (37), intravitreal (38), intranasal (39), and intrathecal (40) administration.”
Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation (2017, neutral): “A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript REAGENT or RESOURCE SOURCE IDENTIFIER pCAG-GFP Matsuda and Cepko, 2004 Addgene plasmid: 11150 pPGK-H2B-mCherry-2A-DHB(aa994-1087)-GFP From Dr. Sergi Regot (Spencer et al., 2013) N/A Software and Algorithms Image J NIH…”
Genomic Analysis of Mouse Retinal Development (2004, background): “The use of in vivo electroporation (Matsuda and Cepko 2004) and plasmid constructs encoding small inhibitory RNAs delivered by electroporation or retroviruses will make possible medium-throughput gainand loss-of-function studies of gene function in the retina.”