Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides

By Hamilton O. Smith, C. Hutchison, C. Pfannkoch, J. Venter. Proceedings of the National Academy of Sciences of the United States of America, 2003

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Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides (2009, result): “This is comparable to previously reported data obtained with in vitro protocols ( 25 ,26).”
Synthetic Biology: Social and Ethical Challenges (2008, background): “…Development In 2003 Craig Venter, the somewhat infamous genome pioneer, caused a controversy about synthetic biology when he announced that his team had taken just fourteen days to create the first synthetic virus from scratch, bacteriophage Phi X174 (5,386 bp) [Smith et al 2003].”
Enzymatic Assembly of Overlapping DNA Fragments (2011, positive): “For example, oligos can be joined by polymerase cycling assembly and subsequently amplified by PCR (Smith et al., 2003; Stemmer et al., 1995).”
A Synthetic Genetic Edge Detection Program (2009, background): “The construction of very large fragments of DNA (Cello et al., 2002; Chan et al., 2005; Endy, 2008; Gibson et al., 2008a; Gibson et al., 2008b; Smith et al., 2003; Tumpey et al., 2005) is no longer a limitation in the engineering of biological systems.”