Assessing Plant Genetic Diversity by Molecular Tools

By L. Mondini, A. Noorani, M. Pagnotta. 2009

454 citations recorded by the source corpus.

This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation and use of plant genetic resources. Molecular techniques have been applied in the analysis of specific genes, as well as to increase understanding of gene action, generate genetic maps and assist in the development of gene transfer technologies. Molecular techniques have also had critical roles in studies of phylogeny and species evolution, and have been applied to increase our understanding of the distribution and extent of genetic variation within and between species. These techniques are well established and their advantages as well as limitations have been realized and described in this work. Recently, a new class of advanced techniques has emerged, primarily derived from a combination of earlier, more basic techniques. Advanced marker techniques tend to amalgamate advantageous features of several basic techniques, in order to increase the sensitivity and resolution to detect genetic discontinuity and distinctiveness. Some of the advanced marker techniques utilize newer classes of DNA elements, such as retrotransposons, mitochondrial and chloroplast based microsatellites, thereby revealing genetic variation through increased genome coverage. Techniques such as RAPD and AFLP are also being applied to cDNA-based templates to study patterns of gene expression and uncover the genetic basis of biological responses. The most important and recent advances made in molecular marker techniques are discussed in this review, along with their applications, advantages and limitations applied to plant sciences.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ab1c68bf6bbd4d8e1e1fa839508a501474cfb68e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Y. El-Kassaby
2. I. Porth

In Molecular Biology

1. Y. El-Kassaby
2. G. Chung
3. I. Porth

In Plant Science

1. Y. El-Kassaby
2. G. Chung
3. I. Porth

Citations in the corpus

• DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing (2018, background): “throughout genome, highly reproducible and having ability to detect higher level of polymorphism [10].”
• Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives (2015, background): “However, the development of microsatellites requires extensive knowledge of DNA sequences, and sometimes they underestimate genetic structure measurements; hence they have been developed primarily for agricultural species, rather than wild species [39].”
• Assessment of genetic diversity in crop plants - an overview (2017, methodology): “Many reviews have been written focusing on vital issues like changes in genetic diversity under plant breeding [1], genetic vulnerability of modern crop cultivars [2], conservation and utilization of genetic resources [3,4] assessment of genetic diversity using molecular markers [5] and measurement ”
• Assessment of the Genetic Diversity in Forest Tree Populations Using Molecular Markers (2014, background): “It is also clear that variation at selectively neutral molecular markers commonly used to assess genetic diversity within or among populations may not covary with the phenotypic expression of a particular qualitative or quantitative trait of interest [29], such that population differentiation for ad”