Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method.

By K. Casciotti, Daniel Sigman, M. Hastings, J. Böhlke, A. Hilkert. Analytical Chemistry, 2002

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THE MECHANISM OF ISOTOPE FRACTIONATION DURING ALGAL NITRATE ASSIMILATION AS ILLUMINATED BY THE 15N/14N OF INTRACELLULAR NITRATE 1 (2004, methodology): “…of the medium and internal pool nitrate used a recently developed method for nitrate isotopic analysis that uses denitrifying bacteria that lack an active N2O reductase to convert nitrate (and nitrite) to N2O, followed by isotopic analysis of the N2O (Sigman et al. 2001, Casciotti et al. 2002).”
Methods for measuring denitrification: diverse approaches to a difficult problem. (2006, background): “It is only with the recent advent of methodology suitable for seawater samples (Casciotti et al. 2002) that the coupled enrichment in 18O and 15N in the residual NO3 can be studied and such data are appearing rapidly in the literature.”
Assimilation of upwelled nitrate by small eukaryotes in the Sargasso Sea (2011, methodology): “The isotopic composition of the nitrous oxide was measured by gas chromatography-isotope ratio mass spectrometry using a modified ThermoFinnigan GasBench II and DeltaPlu”