Metabolic Mechanism Investigation of Antibacterial Active Cysteine-Conjugated Gold Nanoclusters in Escherichia coli

By Ting-Kuang Chang, T. Cheng, Hsueh‐Liang Chu, Shih-Hua Tan, Jui-Chi Kuo, P. Hsu, Chen-Yen Su, Hui-Min Chen, Chi-Ming Lee, Tsung-Rong Kuo. ACS Sustainable Chemistry and Engineering, 2019

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Elucidating the metabolic mechanism of gold nanoclusters (AuNCs) in bacteria will play a pivotal role in bacterial detection and inhibition. A facile method to investigate the metabolic mechanism of AuNCs is demonstrated in this work. The cysteine-conjugated gold nanoclusters (Cys-AuNCs) were successfully prepared with orange-red fluorescence, high water solubility, and superior biocompatibility by one-pot green synthesis to determine bacterial metabolism. The suggested metabolic process was that Cys-AuNCs were metabolized by Escherichia coli, as verified through a decrease in the fluorescence intensity that was clearly detected at 30 min, indicating the breakage of cysteine on Cys-AuNCs, which was further confirmed via X-ray photoelectron spectroscopy (XPS) that was used to observe the decrease in the size of Cys-AuNCs after being metabolized. The metabolic kinetics of Cys-AuNCs was determined by fitting the change in the fluorescence of Cys-AuNCs as a function of incubation time with E. coli, in which t...

Subfields: Electronic, Optical and Magnetic Materials, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/a81e03d538d5127f8c5be37d25bcaae505453df6