Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo

By L. Maene, P. Debergh. Plant Cell Tissue and Organ Culture, 2004

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Temporary immersion systems in plant micropropagation (2002, background): “…involves periodic transfers of plant material to fresh media, after subcul- tures of 4–6 weeks, due to exhaustion of the nutrients in the medium and also because of continuous tissue growth and proliferation, which is rapidly limited by the size of the culture container (Maene and Debergh, 1985).”