Factors affecting success of PCR amplification of microsatellite loci from otter faeces

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Characterization of target nuclear DNA from faeces reduces technical issues associated with the assumptions of low-quality and quantity template (2007, unknown): “Previous studies have demonstrated that winter-collected faeces provide higher quality DNA than samples collected in other seasons (Lucchini et al. 2002; Piggott and Taylor 2003; Piggott 2004; Maudet et al. 2004; Hájková et al. 2006).”
Ageing and environmental factors affect PCR success in wolf (Canis lupus) excremental DNA samples (2007, background): “from the same species (e.g. DNA extracted from Eurasian otter, Lutra lutra, anal gels performed better than DNA from scats; Hájková et al. 2006); (ii) in different species (e.”
The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring (2010, methodology): “…behaviour often shows seasonality and there is evidence that winter is the best period to collect otter spraints (Kruuk 1992) and that the microsatellite DNA analysis of otter faeces is most successful in cold months, when spraints are collected in the early morning (Hájková et al. 2006).”