Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films
By Suman Singh, P. Solanki, M. Pandey, B. Malhotra. 2006
174 citations recorded by the source corpus.
Cholesterol oxidase, cholesterol esterase and peroxidase have been co-immobilized onto electrochemically prepared polyaniline films. These polyaniline–enzyme films characterized using spectroscopic techniques, have been used to fabricate a cholesterol biosensor. This polyaniline-based cholesterol biosensor has a response time of about 240 s, an apparent Km value as 75 mg dl−1 and can be used to estimate cholesterol concentration up to 500 mg dl−1. These polyaniline/cholesterol oxidase/cholesterol esterase films have a detection limit of 25 mg dl−1 with sensitivity of 0.042 μA mg dl−1. The enzyme films were found to be thermally stable up to 48 °C and have a shelf-life of about 6 weeks when stored at 4 °C. The values of the activation energy before and after the critical temperature were found to be 191 and 95.5 kJ mol−1, respectively.
Subfields: Electrical and Electronic Engineering, Electrochemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/a6e4e08e8a574000916d49f432c2cf56d3342148
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In Electrical and Electronic Engineering
Citations in the corpus
- Biosensing Applications of Polyaniline (PANI)-Based Nanocomposites: A Review (2020, background): “042 mAmg dL (1), and the detection limit of 25mg dL (1):([215])”
- Recent progress in nanobiocatalysis for enzyme immobilization and its application (2014, unknown): “been developed with aim of medical diagnosis [54, 55], medical purposes [3,56] and environmental monitoring [57,58], and the quantification of specific components in”