Random-access scanning microscopy for 3D imaging in awake behaving animals

By K. Nadella, H. Roš, C. Baragli, V. A. Griffiths, G. Konstantinou, Theo Koimtzis, Geoffrey J. Evans, Paul A. Kirkby, R. A. Silver. 2016

133 citations recorded by the source corpus.

Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of neocortical pyramidal cells in awake behaving mice.

Subfields: Biomedical Engineering, Biophysics

Citations in the corpus

• Re-evaluating circuit mechanisms underlying pattern separation (2019, neutral): “Several promising new imaging technologies that can record intracellular calcium from large numbers of identified cells have also been recently developed (Grewe et al., 2011; Katona et al., 2012; Froudarakis et al., 2014; Nadella et al., 2016; Sofroniew et al., 2016; Yang et al., 2016).”