Synthesis, deprotection, analysis and purification of RNA and ribozymes.

By F. Wincott, A. Direnzo, C. Shaffer, S. Grimm, D. Tracz, C. Workman, D. Sweedler, Carolyn Gonzalez, S. Scaringe, N. Usman. 1995

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

The RIG-I receptor adopts two different conformations for distinguishing host from viral RNA ligands (2022, positive): “Oligonucleotides were deprotected and gel purified as previously described (Wincott et al., 1995), and evaluated for purity by mass spectrometry (Novatia).”
RNA binding activates RIG-I by releasing an autorepressed signaling domain (2019, methodology): “U55 and 5′-OH RNAs were synthesized using standard phosphoramidite chemistry and deprotected as previously described (48).”
The Thermodynamic Basis for Viral RNA Detection by the RIG-I Innate Immune Sensor (2012, methodology): “The oligonucleotides were deprotected and gel-purified by methods described previously (17).”
Selective RNA targeting and regulated signaling by RIG-I is controlled by coordination of RNA and ATP binding (2016, methodology): “The hydroxylated oligonucleotides were deprotected and gel purified as previously described (34).”
Establishing the role of ATP for the function of the RIG-I innate immune sensor (2015, methodology): “The oligonucleotides were deprotected and gel purified as previously described (Wincott et al., 1995).”