Single-molecule studies of DNA mechanics.

By Carlos Bustamante, Steven B. Smith, J. Liphardt, Doug Smith. Current Opinion in Structural Biology, 2000

778 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a5ffce5d6e1c7c28f1b6f33e5d4e9d6f0cef13ff

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In Atomic and Molecular Physics, and Optics

In Biomedical Engineering

In Molecular Biology

Citations in the corpus

Nonequilibrium fluctuations in small systems: From physics to biology (2007, background): “In this way it is possible to investigate the mechanical and elastic properties of the DNA molecule [80, 81].”
How do site-specific DNA-binding proteins find their targets? (2004, background): “The key technology for this is the micromanipulation of single, long (tens of kilobase) DNA molecules (73,74).”
Real-time imaging of DNA loop extrusion by condensin (2018, positive): “The dependence of the extrusion rate on the DNA end-to-end length can be understood as a force dependence: As the loop is extruded, the amount of DNA between the two attachment points (excluding the loop) decreases correspondingly, thus continuously increasing the tension within the DNA (19) (to 1.”
Colloquium: The quest for high-conductance DNA (2004, methodology): “There are other possible DNA structures that may result from mechanical stretching, for example, due to the use of the molecular combing technique (Parra and Windle, 1993; Bensimon et al., 1995; Allemand et al., 1997; Bustamante et al., 2000).”
Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, background): “Technological advances over the last two decades made it possible to stretch, bend and twist a single macromolecule and the corresponding moduli of elasticity have been measured [2210, 2211, 2212, 2213, 2214, 2215, 2216, 2217, 2218, 2219, 2220].”
A revolution in optical manipulation (2003, background): “Biological and medical applications of optical tweezers have been reviewed extensivel”
Cell and molecular mechanics of biological materials (2003, background): “For λ-phage DNA in a solution containing 150 nM Na + , S a 1,000 pN (ref”
Mechanics of biomolecules (2002, background): “…DNA as a thin cylindrical rod of homogeneous isotropic linear elastic material with Young’s modulus Y and radius R ≈ 1 nm (Voet and Voet, 1995) then K = R2Y A= YI kBT = R4ED 4kBT : (9) For -phage DNA in a solution containing 150 nM Na+; Y ≈ 300 MPa and K ≈ 1000 pN (Bustamante et al., 2000).”