Sequence analysis of ITS ribosomal DNA in five Phaeoacremonium species and development of a PCR-based assay for the detection of P. chlamydosporum and P. aleophilum in grapevine tissue

By S. Tegli, G. Surico, E. Bertelli. 2000

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A review of Phaeoacremonium species involved in Petri disease and esca of grapevines (2006, methodology): “Species-specific primers have been developed for the detection of Phaeomoniella chlamydospora (PCL1 + PCL2 and Pch1 + Pch2) (Groenewald et al., 2000; Tegli et al., 2000) and Phaeoacremonium aleophilum (Pal1N + Pal2) (Tegli et al., 2000) from the internal transcribed spacers ITS1 and ITS2 of the…”
Taxonomy and Pathology of Togninia (Diaporthales) and its Phaeoacremonium Anamorphs (2006, methodology): “The genetic variation in populations of Pm. aleophilum has been studied in more detail than that of other species (Péros et al. 2000, Tegli et al. 2000b, Cottral et al. 2001).”
Evaluation of the grapevine nursery propagation process as a source of Phaeoacremonium spp. and Phaeomoniella chlamydospora and occurrence of trunk disease pathogens in rootstock mother vines in Spain (2010, methodology): “PCR product was diluted 1:200 and then, 1 μl was used as DNA template for the secondary PCR using primer pair Pm1-Pm2 for Phaeoacremonium spp. or Pch1-Pch2 for Pa. chlamydospora.”