Characterization of 14 microsatellite markers for genetic analysis and cultivar identification of walnut
By G. Dangl, Keith Woeste, M. Aradhya, A. Koehmstedt, C. Simon, D. Potter, Charles A. Leslie, G. McGranahan. 2005
145 citations recorded by the source corpus.
One hundred and forty-seven primer pairs originally designed to amplify microsatellites, also known as simple sequence repeats (SSR), in black walnut (Juglans nigra L.) were screened for utility in persian walnut (J. regia L.). Based on scorability and number of informative polymorphisms, the best 14 loci were selected to analyze a di- verse group of 47 persian walnut accessions and one J. hindsii (Jepson) Jepson ex R.E. Sm x J. regia hybrid (Paradox) rootstock. Among the 48 accessions, there were 44 unique multi-locus profi les; the accessions with identical profi les appeared to be synonyms. The pairwise genetic distance based on proportion of shared alleles was calculated for all accessions and a UPGMA (unweighted pair group method with arithmetic mean) dendrogram constructed. The results agree well with what is known about the pedigree and/or origins of the genotypes. The SSR markers distinguished pairs of closely related cultivars and should be able to uniquely characterize all walnut cultivars with the exception of budsports. They provide a more powerful and reliable system for the molecular characterization of walnut germplasm than those previously tested. These markers have numerous applications for the walnut industry, including cultivar identifi cation, verifi cation of pedigrees for cultivar and rootstock breeding programs, paternity analysis, and under- standing the genetic diversity of germplasm collections.
Subfields: Endocrinology, Nutrition and Dietetics, Plant Science
View Paper: https://www.semanticscholar.org/paper/a5c15ff345b81c575e6c1411cf9d89cd17c31530
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In Endocrinology
In Nutrition and Dietetics
In Plant Science
Citations in the corpus
- Walnut: past and future of genetic improvement (2018, background): “…Dang et al. 2016 Interspecific hybrids identification Pollegioni et al. 2008, 2009 Genetic diversity/relationships determination Foroni et al. 2005; Dangl et al. 2005; Victory et al. 2006; Robichaud et al. 2006; Wang et al. 2008; Aradhya et al. 2009; Gunn et al. 2010; Karimi et al. 2010; Bai et…”