Suppression Subtractive Hybridization and Differential Screening Reveals Endodormancy-associated Expression of an SVP/AGL24-type MADS-box Gene in Lateral Vegetative Buds of Japanese Apricot

By H. Yamane, Yuki Kashiwa, Tomomi Ooka, R. Tao, K. Yonemori. 2008

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To understand the molecular basis of the endodormancy of buds of perennial plants, we searched for the genes that are expressed preferentially in endodormant lateral buds of the deciduous fruit tree japanese apricot (Prunus mume Sieb. et Zucc.) using suppression subtractive hybridization with mirror orientation selection (SSH/ MOS). We generated two SSH/MOS libraries containing gene pools that are expressed preferentially in endodormant buds in comparison with paradormant or ecodormant buds to search for the genes that are upregulated by endodormancy induction or down-regulated by endodormancy release, respectively. Differential screening and sequencingindicatedthatgenesinvolvedingibberellinmetabolism,stressresistance,cellwallmodification,andsignal transduction, such as transcription factors, are upregulated in endodormant buds. After a further expression survey and full-length cDNA cloning, we found that a gene similar to the SVP/AGL24-type MADS-box transcription factor showedendodormancy-associatedexpression.SeasonalexpressionanalysissuggestedthattheSVP/AGL24homologin japanese apricot might be involved in endodormancy regulation of its lateral buds.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/a589be76b06af20cc98a6fcdd4150d6203e84d14