Spheroid culture as a tool for creating 3D complex tissues.

By Eelco M Fennema, Nicolas Rivron, J. Rouwkema, C. V. van Blitterswijk, J. de Boer. Trends in Biotechnology, 2013

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Modeling Physiological Events in 2D vs. 3D Cell Culture. (2017, methodology): “Another culture method involves spheroids forming within hanging droplets of media (35, 55, 110, 111).”
3D bioactive composite scaffolds for bone tissue engineering (2017, background): “Cell aggregates or spheroids have superior intercellular communication and extracellular matrix development when compared to cells grown in 2D culture, potentially accelerating the growth of printed constructs towards functional tissue after bioprinting [85].”
In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform (2016, methodology): “Overall, MCTS are more resistant to treatment than cells in 2D culture (Lin and Chang, 2008; Mehta et al., 2012; Fennema et al., 2013) and can recapitulate the drug resistance observed in solid tumors (Friedrich et al.”