Cell-free translation reconstituted with purified components

By Y. Shimizu, A. Inoue, Yukihide Tomari, Tsutomu Suzuki, T. Yokogawa, K. Nishikawa, T. Ueda. Nature Biotechnology, 2001

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Paper-based Synthetic Gene Networks (2014, positive): “The commercially available PT7 expression system, which is assembled from ribosomes and 35 purified bacterial proteins (Shimizu et al., 2001; Shimizu andUeda, 2010), was freeze dried to form a pellet, reconstituted inwater, and then used to transcribe and translate a constitutive GFP expression…”