Sperm cryopreservation with supplementation of α-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

By E. Figueroa, J. Farías, Manuel Lee-Estevez, I. Valdebenito, J. Risopatrón, C. Magnotti, J. Romero, I. Watanabe, R. Oliveira. Aquaculture, 2018

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Abstract The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activity and fertilization capacity of frozen Atlantic salmon spermatozoa were studied. Spermatozoa were frozen in Cortland® medium supplemented with 1.3 M DMSO, 0.3 M glucose, 2% BSA and different concentrations of α-tocopherol and ascorbic acid; spermatozoa frozen without antioxidants were used as control and fresh sperm. Samples loaded in 0.5 ml plastic straws were frozen at 68 °C/min (Freeze Control®). Thawing was carried out at 35 °C and post-thawing lipid peroxidation (LPO) was evaluated along with Glutathione (GSH/GSSG) levels; and Glutathione peroxidase (GPx) and Catalase (CAT) activity. Additionally, percentage of motility, mitochondrial membrane potential (ΔΨM), and fertilization rate were assessed. Results showed significantly lower LPO values (expressed as malondialdehyde concentration) in spermatozoa cryopreserved with α-tocopherol (0.1 mM and 0.5 mM) and with combinations of α-tocopherol/ascorbic acid (0.1 mM/1.0 mM and 0.5 mM/10 mM ratios) (p

Subfields: Physiology, Public Health, Environmental and Occupational Health, Reproductive Medicine

View Paper: https://www.semanticscholar.org/paper/a477609e43965babf9f494c5ad5305b2d9913083