Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma.

By M. Castellarin, René L. Warren, J. Freeman, L. Dreolini, Martin Krzywinski, Jaclyn Strauss, Rebecca Barnes, P. Watson, Emma Allen-Vercoe, Richard A. Moore and 1 more. Genome Research, 2012

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An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the fecal microbiota, but have been cultured previously from biopsies of inflamed gut mucosa. We obtained a Fusobacterium isolate from a frozen tumor specimen; this showed highest sequence similarity to a known gut mucosa isolate and was confirmed to be invasive. We verified overabundance of Fusobacterium sequences in tumor versus matched normal control tissue by quantitative PCR analysis from a total of 99 subjects (p = 2.5 × 10(-6)), and we observed a positive association with lymph node metastasis.

Subfields: Infectious Diseases, Molecular Biology, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/a3e544bb4915ec0e503290a0493f656554cecc5c

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Citations in the corpus

The first 1000 cultured species of the human gastrointestinal microbiota (2014, background): “…appendicitis are associated with a local infection of Fusobacterium spp. (Swidsinski et al., 2011), while the increased abundance of Fusobacterium spp. is associated with ulcerative colitis (Rajili c-Stojanovi c et al., 2013a) and colorectal cancer (Castellarin et al., 2012; Kostic et al., 2012).”
Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin (2013, methodology): “Compared to the metagenomic approach in the previous studies (Castellarin et al., 2012; Kostic et al., 2012), qPCR is significantly less time consuming and more cost effective, suitable for clinical purposes.”
The microbiome and human cancer (2021, background): “However, other studies have indeed shown cultivable bacteria in breast (127–129), lung (18), prostate (130, 131), pancreas (14, 15), and colon cancers (19, 132), suggesting broad microbial viability.”
Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy (2017, methodology): “In agreement with our data in Cohort 1 and previous reports (Castellarin et al., 2012; Kostic et al., 2012), the amount of F.”
Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment (2012, result): “Particularly as the former have also been associated with the colorectal cancer microenvironment in previous work [68,69], it is of note that these variations in the microbiota with respect to biogeography and pH are similar to those we observed with respect to IBD and potentially redox status as de”
Association between periodontal pathogens and systemic disease (2019, background): “nucleatum could migrate to and colonize the human intestinal tract, to cause deleterious inflammatory infections [47,49].”