Development and validation of an HPLC-FLD method for rapid determination of histamine in skipjack tuna fish (Katsuwonus pelamis)

By Saeed Tahmouzi, R. Khaksar, M. Ghasemlou. 2011

87 citations recorded by the source corpus.

Abstract This study compared and validated two methods of high-performance liquid chromatography: fluorescence detection (HPLC-FLD) and UV–Vis detection. Recoveries greater than 55% at all spiking levels using UV–Vis detection could not be obtained. However, derivatization with pre-column o -phthalaldehyde (OPA) was found to efficiently enhance the method sensitivity. The analytical method was validated based on the following criteria: linearity, sensitivity, accuracy, precision, repeatability, and recovery. For fluorescence detection, excellent linear correlation with R 2  = 0.9977 was observed over the range of 5.0 to 100.0 mg/L. The minimum detection limit was calculated to be 1.5 mg/kg, while the limit of quantification (LOQ) value of the validated method was determined to be 4.5 mg/kg. Good recoveries ( RSD % = 1.35) were obtained at all spiking levels ranging between 0 and 200 mg/kg. The proposed method was found to be suitable, selective, and rapid for the determination of histamine.

Subfields: Insect Science, Molecular Biology, Pollution

View Paper: https://www.semanticscholar.org/paper/a3a56570d14ebed26d846399e064ecf74a5ca6d0