A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish.

By K. Kawakami, Hisashi Takeda, N. Kawakami, Makoto Kobayashi, Naoto Matsuda, M. Mishina. Developmental Cell, 2004

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Hematopoietic stem cells derive directly from aortic endothelium during development (2010, neutral): “The transgenic construct was then excised and ligated into a Tol2 transgenesis vector 27 .”
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair (2014, background): “Given the wealth of eGFP enhancer and gene trap lines previously generated in zebrafish (Kawakami et al. 2004; Parinov et al. 2004; Ellingsen et al. 2005), this offers new possibilities for deeper analysis of the marked cell types by tissue-specific expression of various UAS-driven constructs.”
The Tol2kit: A multisite gateway‐based construction kit for Tol2 transposon transgenesis constructs (2007, methodology): “3), it was clear that coinjection of transposase RNA increases the number of fluorescent embryos, the number of fluorescent cells in each embryo, and the total fluorescence of each embryo, replicating previous results seen with Tol2 constructs generated by conventional subcloning (Kawakami et al., 2”