An introduction to the wound healing assay using live-cell microscopy
By J. Jonkman, Judith A. Cathcart, Feng Xu, M. Bartolini, Jennifer E Amon, K. Stevens, P. Colarusso. Cell Adhesion & Migration, 2014
637 citations recorded by the source corpus.
Abstract The wound healing assay is used in a range of disciplines to study the coordinated movement of a cell population. In this technical review, we describe the workflow of the wound healing assay as monitored by optical microscopy. Although the assay is straightforward, a lack of standardization in its application makes it difficult to compare results and reproduce experiments among researchers. We recommend general guidelines for consistency, including: (1) sample preparation including the creation of the gap, (2) microscope equipment requirements, (3) image acquisition, and (4) the use of image analysis to measure the gap size and its rate of closure over time. We also describe parameters that are specific to the particular research question, such as seeding density and matrix coatings. All of these parameters must be carefully controlled within a given set of experiments in order to achieve accurate and reproducible results.
Subfields: Biomedical Engineering, Cell Biology, Rehabilitation
View Paper: https://www.semanticscholar.org/paper/a33b07d3009dd10ec25ad15ac1ad52433f53b290
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biomedical Engineering
Citations in the corpus
- An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays (2020, methodology): “With respect to the MRI wound healing tool (which is also based on a variance-based method (19)), our plugin also underestimated the area by 6.”