Gene Regulation with Polyvalent siRNA-Nanoparticle Conjugates

By D. Giljohann, D. Seferos, Andrew E. Prigodich, Pinal C. Patel, Chad A. Mirkin. Journal of the American Chemical Society, 2009

631 citations recorded by the source corpus.

We report the synthesis and characterization of polyvalent RNA-gold nanoparticle conjugates (RNA-Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a3253c8f7f56915a9cff08074f00690a432b8676

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Citations in the corpus

• Functionalized Gold Nanoparticles and Their Biomedical Applications (2011, background): “Polyvalent RNA-GNP conjugates densely functionalized with synthetic RNA oligonucleotides were designed to function in the RNAi pathway [38].”