RNA-Seq: a revolutionary tool for transcriptomics

By Zhong Wang, Mark Gerstein, M. Snyder. Nature reviews genetics, 2009

12,936 citations recorded by the source corpus.

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a2f71b2d0edda572f310d95fe547ff1f51e68d55

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Citations in the corpus

SNP Discovery through Next-Generation Sequencing and Its Applications (2012, background): “RNA deep-sequencing technologies such as digital gene expression [46] and Illumina RNASeq [47] are both qualitative and quantitative in nature and permit the identiﬁcation of rare transcripts and splice variants [48].”
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