In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast

By P. Senthilraja, K. Kathiresan. 2015

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Abstract Marine yeasts isolated from coastal mangrove ecosystem namely Candida albicans, Kuraishia capsulate and Sacchromyces cerevisae were screened for the cytotoxicity against a non-transformed Vero (African green monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 µg/ml).lower doses enhanced the viability of the cultured cells,MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was used to measure the cell proliferation and survival. Of three yeast strains, Sacchromyces cerevisae showed more than 80% cell viability in Vero cell lines and were studied for further cytotoxicity against HepG2, MCF -7 cell lines respectively.

Subfields: Biotechnology, Computational Theory and Mathematics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a1daa37eeff83c7ee01f32563c029542e21e8084