Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum

By D. E. Muscarella, E. L. Ellison, B. Ruoff, V. Vogt. 1990

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Degenerate DNA recognition by I-PpoI endonuclease. (1998, background): “…faster gene encoding the large ribosomal subunit of the slimethan non-cognate sites, and the alteration of a single mold Physarum polycephalum (Muscarella et al., 1990).base within the recognition site is sufficient to cripple In the presence of Mg2+, the enzyme cleaves two phos-the enzyme’s…”
Interaction of the intron-encoded mobility endonuclease I-PpoI with its target site (1993, methodology): “I-PpoI has been expressed in several systems, including rabbit reticulocyte lysates, Escherichia coli (34), and S.”
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site. (2000, methodology): “…and HNH. I-PpoI was the ®rst identi®ed member of the HisCys box homing endonuclease family and is encoded by an open reading frame in the third intron of the nuclear, extrachromosomal 26 S rRNA gene of the slime mold Physarum polycephalum (Muscarella et al., 1990b; Muscarella & Vogt, 1989).”