Inaugural Article: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA

By M. Stiller, R. Green, Michael T. Ronan, J. Simons, Lei Du, Wei He, M. Egholm, Jonathan Rothberg, S. Keates, N. D. Ovodov and 9 more. 2006

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Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations (2018, background): “High-accuracy sequencing methods are especially important when heterogeneous mixed samples are degraded and therefore more error prone, as may occur at both modern crime scenes and with ancient DNA from archaeological sites 192 .”
Detection of ultra-rare mutations by next-generation sequencing (2012, background): “Likewise, the excess C→T mutations are consistent with spontaneous deamination of cytosine to uracil (37), a particularly common DNA damage event that results in insertion during PCR of adenine opposite uracil and erroneous scoring of a C→T mutation.”
Ancient plant DNA in archaeobotany (2008, background): “The very details of the whole process of DNA preservation and the mode of modifications at sequence level are still subject to research (Gilbert et al. 2003, 2007a; Pääbo et al. 2004; Hansen et al. 2006; Stiller et al. 2006).”