Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum)
By G. K. Cho, D. Heath. 2000
232 citations recorded by the source corpus.
This study investigated the feasibility of using clove oil as an alternative to tricaine methanesulphonate as a fish anaesthetic, particularly in fish stress research. The physiological stress responses of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum) anaesthetized with either tricaine (50 mg L−1) or clove oil (20 p.p.m.) were compared using unanaesthetized fish as controls. Haematocrit, serum cortisol and serum glucose concentrations, serum lysozyme activity and differential leucocyte counts were measured from blood samples collected before, during and upon recovery from anaesthesia and at specified intervals up to 72 h after recovery. Differences between the two anaesthetic groups were not significant for most of the physiological traits measured. Serum lysozyme activity of control fish, however, was significantly suppressed relative to the treated fish for 72 h after stress. Clove oil may be a safe and cost-effective alternative to tricaine without significantly affecting study results. Furthermore, clove oil may be more practical for field-based research, because a withdrawal period is unnecessary, and clove oil does not pose an environmental hazard.
Subfields: Animal Science and Zoology, Aquatic Science, Immunology
View Paper: https://www.semanticscholar.org/paper/a12e6b084967d2de13cbb402d1d9da817b3e63bf
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In Animal Science and Zoology
In Aquatic Science
In Immunology
Citations in the corpus
- Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides) (2004, background): “physiological disturbance and minimizes responses to external stressors to that observed with MS 222 (Cho and Heath, 2000; Sladky et al., 2001; Wagner et al., 2003).”
- Effects of anaesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss) (2003, background): “58Several studies describe physiological effects of different anaesthetics (e.g. Soivio et 59al., 1977; Iwama et al., 1989; Cho and Heath, 2000), their efficacy (Keene et al., 1998; 60Munday and Wilson, 1997; Peake, 1998) and effects on swimming performance after 61anaesthesia (Anderson et al.,…”
- Effects of clove oil on the stress response of matrinxã (Brycon cephalus) subjected to transport (2005, background): “…the FDA does not approve the use of clove oil for aquaculture purposes (Sladky et al., 2001) Researchers have investigated the effects of clove oil in fishes (Soto & Burhanuddin, 1995; Keene et al., 1998; Cho & Heath, 2000; Sladky et al., 2001; Tort et al., 2002; Woody et al., 2002; Small, 2004).”