A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy
By Xiangliang Pan, Jing Liu, Daoyong Zhang, Xi Chen, Lan-hai Li, Wenjuan Song, Jianying Yang. 2010
93 citations recorded by the source corpus.
Two physical methods (centrifugation and ultrasonication) and 3 chemical methods (extraction with EDTA, extraction with formaldehyde, and extraction with formaldehyde plus NaOH) for extraction of EPS from alga-bacteria biofilm were assessed. Pretreatment with ultrasound at low intensity doubled the EPS yield without significant modification of the composition of EPS. Extraction with EDTA or extraction with formaldehyde plus NaOH increased yield by about 1 order of magnitude compared with other methods. However, the protein and polysaccharide content in EPS prepared with EDTA or formaldehyde plus NaOH were low. Two fluorescence peaks belonging to protein-like peaks and 2 fluorescence peaks belonging to humic acid-like substances were found in 3DEEM fluorescence spectra of all the EPS samples prepared using different methods. Fulvic-like fluorescence was detected only in the EPS extracted with formaldehyde plus NaOH. Location of, and fluorescence intensity at, each peak were clearly affected by the extraction methods. Dialysis was also found to be an important factor influencing the yield, composition and fluorescence characteristics of EPS.
Subfields: Geochemistry and Petrology, Health, Toxicology and Mutagenesis, Pollution
View Paper: https://www.semanticscholar.org/paper/9fdbf84cf15f1235ef6a45448d8d9f1001d528c4
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In Health, Toxicology and Mutagenesis
Citations in the corpus
- Critical review on biofilm methods (2017, methodology): “…or lyophilization) and/or chemical reagents (ethanol, formaldehyde, formamide, NaOH, EDTA or glutaraldehyde) (Adav & Lee, 2008; Azeredo et al., 1998; Brown & Lester, 1980; Chu et al., 2015; Comte et al., 2006; Gong et al., 2009; Kunacheva & Stuckey, 2014; Pan et al., 2010; Tapia et al., 2009).”