Bioprinted Amniotic Fluid-Derived Stem Cells Accelerate Healing of Large Skin Wounds
By A. Skardal, D. Mack, E. Kapetanovic, A. Atala, John D. Jackson, J. Yoo, S. Soker. Stem Cells Translational Medicine, 2012
595 citations recorded by the source corpus.
Stem cells obtained from amniotic fluid show high proliferative capacity in culture and multilineage differentiation potential. Because of the lack of significant immunogenicity and the ability of the amniotic fluid‐derived stem (AFS) cells to modulate the inflammatory response, we investigated whether they could augment wound healing in a mouse model of skin regeneration. We used bioprinting technology to treat full‐thickness skin wounds in nu/nu mice. AFS cells and bone marrow‐derived mesenchymal stem cells (MSCs) were resuspended in fibrin‐collagen gel and “printed” over the wound site. At days 0, 7, and 14, AFS cell‐ and MSC‐driven wound closure and re‐epithelialization were significantly greater than closure and re‐epithelialization in wounds treated by fibrin‐collagen gel only. Histological examination showed increased microvessel density and capillary diameters in the AFS cell‐treated wounds compared with the MSC‐treated wounds, whereas the skin treated only with gel showed the lowest amount of microvessels. However, tracking of fluorescently labeled AFS cells and MSCs revealed that the cells remained transiently and did not permanently integrate in the tissue. These observations suggest that the increased wound closure rates and angiogenesis may be due to delivery of secreted trophic factors, rather than direct cell‐cell interactions. Accordingly, we performed proteomic analysis, which showed that AFS cells secreted a number of growth factors at concentrations higher than those of MSCs. In parallel, we showed that AFS cell‐conditioned media induced endothelial cell migration in vitro. Taken together, our results indicate that bioprinting AFS cells could be an effective treatment for large‐scale wounds and burns.
Subfields: Genetics, Radiology, Nuclear Medicine and Imaging, Rehabilitation
View Paper: https://www.semanticscholar.org/paper/9f8481a23f65fd61ac95f933e0087d721e640262
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Genetics
Citations in the corpus
- Burn injury: Challenges and advances in burn wound healing, infection, pain and scarring (2018, methodology): “Various biomaterials have been evaluated for bioprinting skin such as collagen [288], bovine gelatin [289], chitosan and silk fibrin [290], also synthetic biopolymers being PCL, poly (lactic acid) PLA, poly (lactide-coglycolide) PLGA and PLLA [291] or human plasma [292].”
- A new chapter in pharmaceutical manufacturing: 3D‐printed drug products☆, ☆☆ (2017, background): “Although printing on patients sounds fanciful, extrusion and jetting techniques have been applied to create tissue engineering scaffolds [55] and wound-healing gels [120] on-demand.”
- Current advances and future perspectives in extrusion-based bioprinting. (2016, background): “Although collagen type I has some disadvantages, such as sensitivity to metalloproteinases and poor mechanical properties [128], it has been successfully used as a major biomaterial supporting other hydrogels such as fibrin [129].”