Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
By A. Azam, A. S. Ahmed, Mohammad Oves, M.S.A. Khan, A. Memić. International Journal of Nanomedicine, 2012
748 citations recorded by the source corpus.
Background CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species. Here we synthesized different sized CuO nanoparticles and explored the size-dependent antibacterial activity of each CuO nanoparticles preparation. Methods CuO nanoparticles were synthesized using a gel combustion method. In this approach, cupric nitrate trihydrate and citric acid were dissolved in distilled water with a molar ratio of 1:1. The resulting solution was stirred at 100°C, until gel was formed. The gel was allowed to burn at 200°C to obtain amorphous powder, which was further annealed at different temperatures to obtain different size CuO nanoparticles. We then tested the antibacterial properties using well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration methods. Results XRD spectra confirmed the formation of single phase CuO nanoparticles. Crystallite size was found to increase with an increase in annealing temperature due to atomic diffusion. A minimum crystallite size of 20 nm was observed in the case of CuO nanoparticles annealed at 400°C. Transmission electron microscopy results corroborate well with XRD results. All CuO nanoparticles exhibited inhibitory effects against both Gram-positive and -negative bacteria. The size of the particles was correlated with its antibacterial activity. Conclusion The antibacterial activity of CuO nanoparticles was found to be size-dependent. In addition, the highly stable minimum-sized monodispersed copper oxide nanoparticles synthesized during this study demonstrated a significant increase in antibacterial activities against both Gram-positive and -negative bacterial strains.
Subfields: Materials Chemistry, Water Science and Technology
View Paper: https://www.semanticscholar.org/paper/9f1a73966717a4c08858832bf967dfe4daa549d2
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- Mohammad Oves
- M. S. Khan
- A. Azam
- A. S. Ahmed
- S. Habib
- A. Memić
- M. Ahemad
- A. Zaidi
Citations in the corpus
- Synthesis and Antibacterial Activity of CuO Nanoparticles Suspension Induced by Laser Ablation in Liquid (2015, background): “The bactericidal property of such NPs depends on their size, stability, and concentration added to the growth medium, which provides greater retention time for bacterium NP interaction [16,17].”
- Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study (2012, positive): “Similar activity observations have been made for nanoparticles composed of a single metal oxide.(6,7,10) However, it should also be noticed that Gram-negative bacterial strains of E.”
- Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application (2013, neutral): “45 In the present investigation, a higher inhibition zone was observed for the Gram-positive S. aureus compared to other Gram-negative strains employed in this antibacterial susceptibility assay.”