A single band does not always represent single bacterial strains in denaturing gradient gel electrophoresis analysis

By Hiroyuki Sekiguchi, N. Tomioka, T. Nakahara, H. Uchiyama. Biotechnology Letters, 2001

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Nucleic acid-based approaches to investigate microbial-related cheese quality defects (2012, background): “Sekiguchi et al., found that a single DGGE band contained several different sequences (Sekiguchi et al., 2001).”
MULTI‐GROUP BIODIVERSITY IN SHALLOW LAKES ALONG GRADIENTS OF PHOSPHORUS AND WATER PLANT COVER (2005, background): “…interpretation of these banding patterns in terms of taxon richness may be confounded by the fact that single bands may contain DNA from several species (Sekiguchi et al. 2001) and because several bands may be generated from a single species (Speksnijder et al. 2001), these interferences are rare.”
Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. (2004, methodology): “This is not unusual [45], and to resolve these sequences either cloning or running the fragment on new DGGE can be employed.”
Effects of phosphorus enrichment and grazing snails on modern stromatolitic microbial communities (2005, background): “More intense bands may indicate a higher relative abundance of that ribotype in the overall community (Brüggemann et al., 2000; Abed & Garcia-Pichel, 2001; Sekiguchi et al., 2001; Abed et al., 2002).”