Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta.

By Rainer Waadt, Lena K Schmidt, Marc Lohse, Kenji Hashimoto, Ralph Bock, J. Kudla. The Plant Journal, 2008

671 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9d08f0ecb0a81fe21d788453cf6e49034196857c

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In Molecular Biology

1. Tetsuya Kimura
2. Xiaoyan Dai
3. M. Matz
4. Sergey A. Lukyanov
5. T. Nakagawa
6. Konstantin A. Lukyanov
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In Plant Science

1. S. Luan
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4. Tetsuya Kimura
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7. T. Nakagawa
8. Jocelyn K. C. Rose

Citations in the corpus

• Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction (2012, methodology): “Although there are alternative positions to split a fluorescent protein into two fragments (Hu & Kerppola, 2003; Waadt et al., 2008), the CFP, GFP and YFP in our system were split between residues 174 and 175, and mRFP1, which contains an amino acid substitution of the 66th glutamine to threonine, w”
• COLD1 confers chilling tolerance in rice. (2015, positive): “Bimolecular Fluorescence Complementation BiFC experiments and gene transformation were performed as described previously (Stagljar et al., 1998; Waadt et al., 2008; Wang et al., 2009).”
• Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “A number of efficient split variants were developed for blue (EBFP), cyan (ECFP, Cerulean, SCFP3A), green (EGFP), and yellow (EYFP, Venus, Citrine) mutants of Aequorea victoria GFP (178, 179, 398, 462).”
• CHL1 functions as a nitrate sensor in plants. (2009, methodology): “As described previously (Waadt et al., 2008), mesophyll protoplasts were isolated from 2-week-old wild-type plants (ecotype Columbia) and transformed with different YFP fusion protein combinations (http://genetics.mgh.harvard. edu/sheenweb/protocols_reg.html), and then, after incubation at 23 C for…”
• A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells (2009, methodology): “To generate the BiFC constructs, SLAC1, OST1, and PP2CA full length cDNA with no stop codon were subcloned via SpeI/SalI into 35S-SPYNE(R)173 and 35S-SPYCE(M) vectors (42).”